The Netherlands Brain Bank (NBB) is a department of the NIN and collects brain tissue of brain donors that registered during life. Brain donors and tissue are well characterized and disseminated open access to researchers worldwide. Recently the NBB started ‘The Netherlands Neurogenetics Database” (NND) project in which clinical, pathological and genetic donor data are prepared for analysis to determine polygenic risk scores for brain diseases and facilitate donor selection for research. The Neuroimmunology Research (IMM) group in the NIN, led by Prof. Inge Huitinga, studies the innate and adaptive immune responses in multiple sclerosis (MS) and psychiatric diseases using postmortem human brain tissue provided by the NBB.
The tasks overall concern the assistance of the analysis of post-mortem human brain tissue in all aspects:
- Gene expression analysis (qPCR, RNAseq)
- DNA isolation for GWAS and whole genome sequencing
- Immunohistochemistry on human post-mortem brain tissue
- Different microscopic techniques (light microscope, CLSM, STED)
- Isolation, in vitro culture and experimental analysis of primary human glia cells
- Isolation and analysis of primary cells and nuclei from frozen tissue
- Strong interest in human brain research
- Knowledge of and experience with immunohistochemistry, microscopy (light, fluorescence, STED) and molecular techniques such as RNA and DNA isolation, qPCR and RNA scope
- Team player
- Highly accurate
- Contract of employment of 2 years
Depending on education and relevant work experience, the gross monthly salary based on fulltime employment varies between € 2.067 and € 2.791 gross per month (salary scale 7, 1.0 fte), in accordance with the Collective Labour Agreement for Dutch Universities (CAO NU), excluding 8% holiday allowance and 8.3% end-of-year bonus. There are excellent fringe benefits.
Application and deadline
Applicants are requested to write a letter in which they motivate their application, accompanied by a curriculum vitae and one or two references. Applications should be sent before August 1st, 2020, to the Netherlands Institute for Neuroscience, attn. of Prof.dr. I Huitinga (email@example.com), with reference “vacancy research technician NDD/IMM”. Evaluation of candidates will begin immediately and will continue until the vacancy is filled.
Any other correspondence in response to this advertisement will not be dealt with