Maarten KoleNeuroscience Symposium
The Neuroscience Symposia are organized weekly by the Netherlands Institute for Neuroscience. The presentations are given by researchers from the institute or by guest speakers. The title and content of the symposium is usually made known in the week prior to the presentation.
Colloquium room – Netherlands Institute for Neuroscience
4pm – Maarten Kole: Demyelination-induced hippocampal synapse loss affects social memory
Koen Kole : Cell type- and myelin-dependent subcellular distribution of axonal mitochondria
5pm – Followed by drinks
Cognitive impairments represent a major burden in multiple sclerosis (MS) and may involve the temporal lobe and hippocampal circuits. In this talk I will review data from immunofluorescence, electrophysiological recordings together with behavioral experiments showing that in the demyelinated CA2 subfield in mice the complement complex C1q is associated with microglia-mediated pruning of inhibitory connections and impacts CA2-related memory. Comparative analyses in MS post-mortem tissue confirmed a hippocampal subfield-specific pattern. The results reveal an emerging role of the CA2 subfield in the demyelinated hippocampus.
Axonal mitochondria receive energy substrates in part via the myelin sheath, enabling local ATP synthesis. It is currently unknown whether mitochondria respond to demyelination in a cell type-specific manner. During this symposium, I will discuss a Cre-dependent AAV approach that we developed to fluorescently label mitochondria in molecularly defined cell types. The results show high specificity of mitochondrial labeling in cortical layer 5 pyramidal neurons (Rbp4-Cre mice) or parvalbumin expressing (PV+) interneurons (PV-Cre mice), yielding new insights into the subcellular characterization of mitochondria in the healthy and demyelinated cortex.