Support our work
Decorative header background

HCN channel-mediated neuromodulation can control action potential velocity and fidelity in central axons

Research group Kole
Publication year 2019
Published in eLife
Authors Niklas Byczkowicz, Abdelmoneim Eshra, Jacqueline Montanaro, Andrea Trevisiol, Johannes Hirrlinger, M.H.P. Kole, Ryuichi Shigemoto, Stefan Hallermann

Hyperpolarization-activated cyclic-nucleotide-gated (HCN) channels control electrical rhythmicity and excitability in the heart and brain, but the function of HCN channels at the subcellular level in axons remains poorly understood. Here, we show that the action potential conduction velocity in both myelinated and unmyelinated central axons can be bidirectionally modulated by a HCN channel blocker, cyclic adenosine monophosphate (cAMP), and neuromodulators. Recordings from mouse cerebellar mossy fiber boutons show that HCN channels ensure reliable high-frequency firing and are strongly modulated by cAMP (EC50 40 µM; estimated endogenous cAMP concentration 13 µM). In addition, immunogold-electron microscopy revealed HCN2 as the dominating subunit in cerebellar mossy fibers. Computational modeling indicated that HCN2 channels control conduction velocity primarily by altering the resting membrane potential and are associated with significant metabolic costs. These results suggest that the cAMP-HCN pathway provides neuromodulators with an opportunity to finely tune energy consumption and temporal delays across axons in the brain.

Support our work!

The Friends Foundation facilitates groundbreaking brain research. You can help us with that.

Support our work