The NIN researchers Fred de Winter and Joost Verhaagen contributed to a study of the laboratory of Harold MacGillavry (Department of Biology, Faculty of Science, Utrecht University). In this study, ORANGE was developed: an Open Resource for the Application of Neuronal Genome Editing. This technology, based on CRISPR/CAS, mediates targeted genomic integration of epitope tags in rodent dissociated neuronal culture, in organotypic slices, and in vivo. ORANGE enables quantification of expression, distribution, and dynamics for virtually any protein in neurons at nanoscale resolution and will be an invaluable tool for real time protein dynamic studies in neurons.
The article can be read here.