Support our work
Decorative header background

A Free-Choice High-Fat High-Sugar Diet Alters Day-Night Gene Expression in Reward-Related Brain Areas in Rats

Research group Kalsbeek
Publication year 2018
Published in Frontiers in Endocrinology
Authors Aurea Susana Blancas-Velazquez, U.A. Unmehopa, Leslie Eggels, Laura L. Koekkoek, A. Kalsbeek, Jorge Mendoza, S.E. La Fleur

Under normal light-dark conditions, nocturnal rodents consume most of their food during the dark period. Diets high in fat and sugar, however, may affect the day-night feeding rhythm resulting in a higher light phase intake. In vitro and in vivo studies showed that nutrients affect clock-gene expression. We therefore hypothesized that overconsuming fat and sugar alters clock-gene expression in brain structures important for feeding behavior. We determined the effects of a free-choice high-fat high-sugar (fcHFHS) diet on clock-gene expression in rat brain areas related to feeding and reward and compared them with chow-fed rats. Consuming a fcHFHS diet for 6 weeks disrupted day-night differences in Per2 mRNA expression in the nucleus accumbens (NAc) and lateral hypothalamus but not in the suprachiasmatic nucleus, habenula, and ventral tegmental area. Furthermore, short-term sugar drinking, but not fat feeding, upregulates Per2 mRNA expression in the NAc. The disruptions in day-night differences in NAc Per2 gene expression were not accompanied by altered day-night differences in the mRNA expression of peptides related to food intake. We conclude that the fcHFHS diet and acute sugar drinking affect Per2 gene expression in areas involved in food reward; however, this is not sufficient to alter the day-night pattern of food intake.

Support our work!

The Friends Foundation facilitates groundbreaking brain research. You can help us with that.

Support our work